Reaction of Silk Fibroin with Diazomethane
نویسنده
چکیده
The treatment of silk with an ethereal solution of diazometbane result ed in a rapid decrease in tyrosine content, with no appreciable d ecrease in tensile strength <o f the fibers . The results indicate that the hydroxyl groups of tyrosine in the 'Untreated fibers are free. The total methoxyl content after treatment of the fiber for 25 hours or longer was greater than could be accounted for by assuming that only phenolic hydroxyl groups had reacted. The "extra" methoxyl was not on either the primary amino groups or on the hydroxyl groups of the amino acid serine, but a.ppears to have resulted from t he reaction of diazomethane with .groups, tentatively assumed to be carboyxl, to form the methyl esters.
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